All
invertebrates require to be preserved in different ways depending
on their stage of life or body structure. Some species also require
them to be collected or 'fixed' in certain ways prior to permanent
storage. The basic recommendations for preserving insects and their
allies are outlined below, but for more detailed information refer
to the book by Upton
(1991).
Includes
soft bodied adults or larvae and hard bodied invertebrates
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Araneae
(spiders): to be preserved in 80% ethyl
alcohol where available or in isopropyl
alcohol. Top
Scorpionida
(scorpions): to be preserved in 80% ethyl alcohol where available
or in isopropyl alcohol. Top
Pseudoscorpionida
(false scorpions): to be preserved in 70% ethyl alcohol where
available or isopropyl alcohol. These tiny creatures can also be
mounted on microslides with Euparal
or similar resin-based
mountants. Top
Opiliones
(harvestmen): to be preserved in 80% ethyl alcohol where available
or isopropyl alcohol. Top
Acari
(mites): to be preserved in 70% ethyl alcohol where available
or isopropyl alcohol. These tiny creatures can also be mounted on
microslides with water-miscible mountants such as Hoyer's
or with Heinz
(PVA) mounting medium if permanent storage is not required.
Top
Chilopoda
(centipedes): to be preserved in 75% ethyl alcohol where available
or isopropyl alcohol. Top
Diplopoda
(millipedes): to be preserved in 75% ethyl alcohol where available
or isopropyl alcohol. Top
Annelida
(earthworms): to be preserved in 85% ethyl alcohol where available
or isopropyl alcohol. Top
Isopoda
(slaters): to be preserved in 85% ethyl alcohol where available
or isopropyl alcohol. Top
Gastropoda
(slugs and snails): place live slugs and snails into water containing
a few menthol crystals and leave over night. When fully extended
remove and place into approximately 90% ethyl alcohol where available
or isopropyl alcohol. Top
Collembola
(springtails): to be preserved into 95% ethyl alcohol or isopropyl
alcohol. Specimens can also be mounted on microslides with with
water-miscible mountants such as Hoyer's or
with Heinz (PVA)
mounting medium if permanent storage is not required. Top
Diplura:
to be preserved into 75% ethyl alcohol or isopropyl alcohol or mounted
on microslides with Hoyer's or other water-miscible mountants or
with Heinz (PVA) if permanent storage
is not essential. Top
Protura:
to be preserved into 80% ethyl alcohol or isopropyl alcohol or mounted
on microslides with Hoyer's or other water-miscible mountant. Top
Archaeognatha
(bristletails): to be preserved into 80% ethyl alcohol or isopropyl
alcohol. Top
Thysanura
(silverfish): to be preserved into 80% ethyl alcohol or isopropyl
alcohol. Top
Ephemeroptera
(mayflies): preserve nymphs in 80% ethyl alcohol or isopropyl
alcohol with about 5% glycerol.
Adults can be preserved pinned through the centre of the thorax
with the wings spread. Top
Odonata
(dragonflies, damselflies): preserve nymphs in 80% ethyl alcohol
or isopropyl alcohol. Adults can be preserved pinned through the
centre of the thorax with the wings spread and front of forewings
set at right angles to the body. Top
Plecoptera
(stoneflies): preserve nymphs in 80% ethyl alcohol or isopropyl
alcohol. Adults can be preserved pinned through the centre of the
thorax with the wings spread. Top
Blattodea
(cockroaches): preserve pinned through the centre of the thorax.
The wings on the left side may be spread if desired. Large specimens
may need to be gutted. Top
Isoptera
(termites): preserve
nymphs in 80% ethyl alcohol or isopropyl alcohol. Make sure to collect
a series of castes especially soldiers and winged adults as identification
is determined by these individuals. Top
Mantodea
(praying mantids): preserve adults pinned between base of wings,
open the fore tibia
and femur
to about right angles and spread the left hand wings. Nymphs preferably
reared through to maturity. Top
Dermaptera
(earwigs): to be preserved in 75% ethyl alcohol or isopropyl
alcohol. Top
Orthoptera
(grasshoppers, crickets, katydids): preserve adults pinned through
the right side of the thorax with the left hand wings spread. Large
specimens may need to be gutted. Nymphs and very soft bodied individuals
should be preserved in 75% ethyl alcohol or isopropyl alcohol. Top
Phasmatodea
(stick insects): gut larger specimens and preserve pinned through
the base of the mesothorax and bases of the mesothoracic legs. Spread
left wings and fold the antennae back along the body. Ideally nymphs
should be reared through to maturity. Top
Embioptera
(web-spinners): to be preserved in 75% ethyl alcohol or isopropyl
alcohol or mounted on slides with resin based mountants such as
Euparal.
Winged adults can be pinned through the centre of the thorax with
the wings spread. Top
Psocoptera
(booklice): to be preserved in 80% ethyl alcohol or isopropyl
alcohol. May be mounted on slides with a resin
based mountant as required. Top
Phthiraptera
(lice): to be preserved in 80% ethyl alcohol or isopropyl alcohol
or mounted onto slides with a resin based mountant. Top
Hemiptera
(bugs, aphids, scale): due to the variety of Hemipteran types
the preservation techniques vary between groups. Most can be preserved
into 75-80% ethyl alcohol or isopropyl alcohol. Larger adults can
be pinned just right of the centre of the thorax, while smaller
species can be placed on points. Spread wings of some pinned species
such as cicadas and hoppers. Other soft bodied species including
scale, mealybugs or aphids can be mounted onto slides with resin
based mountants. Top
Thysanoptera
(thrips): to be preserved mounted onto slides with a resin based
mountant, spreading the legs, wings and straightening the antennae.
Top
Megaloptera
(alderflies, dobsonflies): to be preserved in 80% ethyl alcohol
or isopropyl alcohol. Some species may be preserved dry and pinned
through the centre of the thorax with the wings spread. Top
Neuroptera
(antlions, lacewings): smaller species to be preserved in 80%
ethyl alcohol or isopropyl alcohol. Others pinned through the right
side of the thorax with the wings spread making sure the body is
well supported. Top
Coleoptera
(beetles, weevils): larvae to be fixed in KAA
or hot water and then preserved in 80% ethyl alcohol or isopropyl
alcohol. Adults should be pinned through the right side of the elytra
towards the front, small species on points. Large numbers of adults
can be stored dry with tissues in tubes. Top
Strepsiptera
(stylopids): to be preserved in 80% ethyl alcohol or isopropyl
alcohol or mounted on slides with a resin based mountant. Winged
males can be pinned or placed on points. Top
Mecoptera
(scorpion-flies): adults should be pinned through the right
of the thorax with wings spread. Care should be taken to fold legs
so they do not break off. Larvae and pupae can be fixed in KAA
and preserved in 80% ethyl alcohol or isopropyl alcohol. Top
Siphonaptera
(fleas): to be preserved in 80% ethyl alcohol or isopropyl alcohol
or mounted on slides using a resin based mountant. Top
Diptera
(flies, mosquitoes): larger adults can be pinned through the
right side of the thorax while smaller specimens pinned onto stages
or cube mounts. Larvae and small specimens preserved in 70% ethyl
alcohol or isopropyl alcohol while some species can be mounted on
slides. Top
Trichoptera
(caddisflies): to be preserved in 80% ethyl alcohol or isopropyl
alcohol or pinned through the right side of the thorax with the
wings spread. Top
Lepidoptera
(moths, butterflies): fix large larvae in KAA and then preserve
in 95% ethyl alcohol or isopropyl alcohol. Smaller larvae can be
fixed in hot water then into 95% ethyl alcohol or isopropyl alcohol.
Adults should be preserved pinned through the thorax with the wings
spread, never store adults in alcohol. Top
Hymenoptera
(ants, bees, wasps): preserve adults in 80% ethyl alcohol or
isopropyl alcohol or pin through the right side of the thorax with
smaller specimens on points. Some species may need to be pinned
obliquely to miss the forelegs. Ants are always mounted on points,
never pinned. Preserve larvae and pupae in 80% ethyl alcohol or
isopropyl alcohol. Top
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